LOCUS       pLV-[hsa-miR-638]        9281 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7202..8062
                     /label=Amp(R)
     promoter        8263..8803
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5807..6041
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6616..7071)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6113..6243
                     /label=SV40 pA
     misc_feature    6244..6457
                     /label=SV40 ori
     misc_feature    5128..5724
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3746
                     /label=intron
     CDS             3793..5118
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3536
                     /label=hsa-mir-638 precursor
BASE COUNT     2153 a      2437 c      2590 g      2101 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc gctggtccaa 
     3121 ctgcaggacg ccttcagctc catcggccag agctgccacc tggacctgcc gcagatcgct 
     3181 gtagtgggcg gccagagcgc cggcaagagc tcggtgctgg agaacttcgt gggccggtga 
     3241 gcgggcgcgg cagggatcgc gggcgggtgg cggcctaggg cgcggagggc ggaccgggaa 
     3301 tggcgcgccg tgcgccgccg gcgtaactgc ggcgcttgcg tgcccgcggc ggggaaccgg 
     3361 acggggtcgg ggaggcgggc cctgtgggac gccctgggca gaggactccc tgcaggggct 
     3421 ccggagcagg cccgggcccc aggggcggtg tcacgggcca gggcgccgta ggacaggagg 
     3481 tgcgctggaa ccctgcggtc catctggtcc cagctttcgt gggtgaagtc tgccatgcta 
     3541 gctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt 
     3601 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct 
     3661 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg 
     3721 ttcaaagttt ttttcttcca tttcaggtgt cgtgaaaact acccctctag agtcgagcta 
     3781 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
     3841 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
     3901 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
     3961 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
     4021 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
     4081 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
     4141 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     4201 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     4261 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     4321 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     4381 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     4441 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     4501 gacccaccgg tcgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 
     4561 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 
     4621 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 
     4681 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 
     4741 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 
     4801 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 
     4861 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 
     4921 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 
     4981 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 
     5041 ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc 
     5101 cgcaagcccg gtgcctgagc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg 
     5161 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     5221 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     5281 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     5341 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     5401 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     5461 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     5521 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 
     5581 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     5641 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     5701 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta 
     5761 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 
     5821 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 
     5881 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 
     5941 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 
     6001 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 
     6061 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 
     6121 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     6181 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     6241 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     6301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     6361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac 
     6421 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 
     6481 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 
     6541 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 
     6601 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     6661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     6721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     6781 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     6841 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     6901 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     6961 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     7021 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 
     7081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 
     7141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
     7201 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 
     7261 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 
     7321 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 
     7381 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 
     7441 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 
     7501 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 
     7561 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 
     7621 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 
     7681 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 
     7741 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 
     7801 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 
     7861 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 
     7921 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 
     7981 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 
     8041 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 
     8101 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
     8161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
     8221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
     8281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 
     8341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 
     8401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 
     8461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 
     8521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 
     8581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 
     8641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 
     8701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 
     8761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 
     8821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 
     8881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 
     8941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 
     9001 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 
     9061 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 
     9121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 
     9181 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg 
     9241 cgcaattaac cctcactaaa gggaacaaaa gctggagctg c 
//
